Circulating Brucella species in wild animals of the Serengeti ecosystem, Tanzania

Abstract Background Brucellosis is a bacterial zoonosis of public health and economic importance worldwide. It affects number domestic animals, wild animals humans. Human brucellosis originates from either livestock or wildlife. The species Brucella circulating in Tanzania largely unknown due to insufficient surveillance. This study was carried out identify found selected wildlife hosts the Serengeti ecosystem. Methodology used total 189 archived samples that were obtained cross-sectional studies previously conducted between 2000 2017 ecosystem Tanzania. Whole blood, serum amniotic fluid collected buffalos, lions, wildebeest, impala, zebra hyena available for DNA extraction. Multiplex polymerase chain reaction B. abortus , melitensis ovis suis (AMOS PCR) quantitative real-time PCR (qPCR) targeting bcsp31 IS 711 genes genus detection targets alkB BMEI 1162 detect strains. Results Out tested, 12 (6.35 %) 22 (11.6 positive AMOS-PCR qPCR, respectively. Most lions (52.6 buffaloes (19.6 %). Other included: wildebeest (13.6 %), impala (4.5 samples, 16 (66.7 identified as other six did not amplify neither nor . Conclusions animal shows testing potential this population. zoonotic detected have been reported humans region. findings suggest that, contact network, some could be reservoirs infections within while others are likely dead-end hosts. One Health control strategies continuous surveillance programs reserved areas should implemented help predicting transmission